Regulatory

Part:BBa_M36156

Designed by: Sohaib Shaikh   Group: Stanford BIOE44 - S11   (2014-10-23)


E.coli Cold Shock Promoter (CSP)

The part behaves relatively similar to the heat shock promoter. In the presence of extreme low temperatures, the promoter sequence of is recognized by the protein CS7.4, which is a cold shock transcriptional activator of the genes gyrA and HNS. Once the CS7.4 binds to the cold shock operon, RNA polymerase is recruited to the cold shock promoter region to begin transcription. In sensor based projects, the cold shock promoter should be placed up stream of the gene that the user intends to transcribe.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None